ABSTRACT
Congenital Chagas disease acquired special importance in Chile after the certification of the control of Triatoma infestans and transmission by blood donors affected with Trypanosoma cruzi. In order to establish adequate protocols for intervention and control in infected mother-neonate pairs in endemic zones of Chagas disease, we present partial results (2005-2008) of a pilot project which is being carried out in the Province of Choapa, IV Region, Chile, whose objectives are: determine the current prevalence of the disease in pregnant women, estimate the incidence of vertical transmission of T. cruzi to newborns, determine the lineages of the parasite present in mothers who do and do not transmit the disease, determine the prevalence of Chagas disease in maternal grandmothers of neonates and study placental histopathology. Preliminary results indicated that in this study period, 3.7 percent of the women who gave birth in the Province have Chagas disease and 2.5 percent of their newborns were infected. The most frequent T. cruzi genotypes found in mothers studied during pregnancy were TCI and TCIId, either alone or in mixed infections. A high percentage (74.3 percent) of the grandmothers studied was infected with the parasite. In 29 placentas from mothers with Chagas disease we observed edema, necrosis, fibrinoid deposits and slight lymphoplasmocyte infiltration. In three placentas we found erythroblastosis and in one of them amastigote forms of T. cruzi; this was one of the cases of congenital infection. The evaluation of the diagnostic and control protocols generated will allow us to determine if it has been possible to modify the natural history of vertical transmission of T. cruzi in Chile.
Subject(s)
Adult , Aged , Aged, 80 and over , Female , Humans , Infant, Newborn , Middle Aged , Pregnancy , Chagas Disease/transmission , Endemic Diseases , Infectious Disease Transmission, Vertical/statistics & numerical data , Trypanosoma cruzi/genetics , Chagas Disease/congenital , Chagas Disease/epidemiology , Chile/epidemiology , Genotype , Infectious Disease Transmission, Vertical/prevention & control , Prevalence , Placenta/parasitology , Placenta/pathologyABSTRACT
Molecular evidence showed 46.2 percent of Trypanosoma cruzi infection in Mepraia spinolai insects from North-Central Chile, which is significantly higher than previous reports of up to 26 percent by microscopic observation. Our results show similar infection levels among nymphal stages, ranging from 38.3 to 54.1 percent, indicating that younger nymphs could be as important as older ones in parasite transmission. A cautionary note must be stressed to indicate the potential role of M. spinolai in transmitting T. cruzi in country areas due to the high infection level detected by molecular analysis.
Subject(s)
Animals , DNA, Protozoan/analysis , Insect Vectors/parasitology , Reduviidae/parasitology , Trypanosoma cruzi/isolation & purification , Chile , Chagas Disease/transmission , Nymph/parasitology , Polymerase Chain Reaction , Trypanosoma cruzi/geneticsABSTRACT
Amplification by the polymerase chain reaction (PCR) of Trypanosoma cruzi kinetoplastic DNA was used to enhance sentitivity in the detection of the parasite in blood, with the ultimate goal of improving the parasitological diagnosis of Chagas' disease in 0-10 year-old infected children. Twenty eight children were evaluated by using xenodiagnosis (XD) and PCR. Whereas XD detected 75,0 percent of the cases PCR was positive in 96,8 percent. The usefulness of the PCR was further investigated in the 28 children who have received specific treatment with nifurtimox. Negativetion of XD after three and six months post treatment respectively. These observations suggest that PCR is the most sensitive and quick technique available for direct detection of T. cruzi in chagasic children and that it can be a very useful tool for the follow-up of infected subjects after specific chemotherapeutical treatment
Subject(s)
Humans , Infant, Newborn , Infant , Child, Preschool , Child , Chagas Disease/diagnosis , Polymerase Chain Reaction , Trypanosoma cruzi/isolation & purification , Chagas Disease/drug therapy , Nifurtimox/pharmacology , Sensitivity and Specificity , Treatment Outcome , Trypanosoma cruzi/pathogenicityABSTRACT
Se describe la variedad ligninolítica Pycnoporus cinnabarinus (Jacq. ex. Fr.) Karst. var. osorninus Burgos var. nov. basándose en que sus características morfológicas difieren claramente de P. cinnabarinus. Además esta variedad tiene una tasa de crecimiento superior a la de P. cinnabarinus. El medio líquido con sulfato de amonio como fuente de nitrógeno estimula la formación de clamidosporas. Las fructificaciones en agar-extracto de malta o medios con aserrín de pino forman tubos con 4 poros/mm